Thermo Fisher Scientific TCR beta Monoclonal Antibody (H57-597), eFluor 450, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 0.25 µg/test

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Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• eFluor 506
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 725
• NovaFluor Blue 800
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 810
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The H57-597 monoclonal antibody reacts with the beta chain of mouse TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and by most peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis depending on assay conditions. This antibody is used as a phenotypic marker for TCR beta-expressing T cells.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.25 µg per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal performance.

Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, emits at 445 nm, and is excited with the violet laser (405 nm). Ensure that the instrument can detect this fluorochrome.

Excitation: 405 nm
Emission: 445 nm
Laser: Violet
Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCRs) discriminate foreign from self-peptides presented by MHC class II molecules, which is essential for adaptive immune responses. Recognition of self-peptides is linked to autoimmune diseases, while mutant self-peptides are associated with tumors. Engagement of TCRs by bacterial superantigens can cause toxic shock syndrome.
Autoantibodies to V beta segments of TCRs have been found in patients with rheumatoid arthritis and systemic lupus erythematosus, potentially modulating inflammatory responses.
Human T cells typically express TCR alpha-beta along with CD4 or CD8, while gamma-delta T cells exhibit distinct antigen recognition and are found primarily in epithelial tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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