
Thermo Fisher Scientific MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), eFluor 450, eBioscience
H-2Kd MHC Class I 항원을 인식하는 SF1-1.1.1 단클론 항체로, eFluor 450 형광체가 결합되어 있음. Flow Cytometry용으로 검증됨. 405 nm excitation, 445 nm emission 특성. 마우스 시료 분석에 적합하며 연구용으로만 사용.
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Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Mouse |
| Host/Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SF1-1.1.1 |
| Conjugate | eFluor™ 450 |
| Excitation/Emission Max | 405/445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_10804635 |
Additional Formats
- APC
- APC-eFluor 780
- Biotin
- Functional Grade
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (b, j, k, p, q, s, v) has not been observed.
Applications Reported:
This antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended usage ≤ 0.5 µg per test (100 µL volume). Cell number range: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.
Fluorophore Information:
eFluor™ 450 is an alternative to Pacific Blue™.
- Excitation: 405 nm
- Emission: 445 nm
- Laser: Violet Laser
Ensure instrument compatibility for detection.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This protein is an MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are key in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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