Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), eFluor 450, eBioscience
인간 PD-L1(B7-H1)에 특이적인 MIH1 클론 단일클론 항체로, eFluor 450 형광 표지되어 흐름세포분석에 최적화됨. Violet 레이저(405 nm)에서 여기, 445 nm에서 방출. 면역조직화학, ELISA 등 다양한 응용 가능. 연구용으로만 사용.
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Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), eFluor 450
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 50 publications |
| ELISA | - | View 1 publication |
| ChIP assay | - | View 1 publication |
| Neutralization | - | View 3 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH1 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574090 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 786
- Functional Grade
- NovaFluor Red 685
- NovaFluor Red 700
- NovaFluor Yellow 700
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. It is expressed on a majority of leukocytes and acts as a ligand for PD-1. Interaction of PD-1 with PD-L1 or PD-L2 results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells at 5 µL (0.5 µg) per test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume, with cell numbers ranging from 10⁵ to 10⁸ cells/test.
Fluorophore Information:
eFluor® 450 is an alternative to Pacific Blue®, excited at 405 nm and emitting at 445 nm. Ensure instrument compatibility with Violet laser detection.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Programmed death receptor ligand 1 (PD-L1, B7-H1) is a B7 family member that binds PD-1, negatively regulating T-cell receptor signaling. PD-L1 engagement decreases TCR-mediated proliferation and cytokine production. Expression is abundant in murine and human cancers and upregulated by IFN-gamma, suggesting a role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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