Thermo Fisher Scientific MFN1 Recombinant Rabbit Monoclonal Antibody (JF0954)
Recombinant rabbit monoclonal antibody targeting human MFN1. Validated for Western blot and flow cytometry. Offers high specificity, sensitivity, and lot-to-lot consistency. Supplied as liquid, 1 mg/mL, purified by Protein A. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0954 |
| Immunogen | Recombinant protein within Human Mitofusin 1 (aa 491–620) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809773 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, and individual clones are screened to select optimal candidates.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism. They may be associated with obesity and apoptosis processes. MFN1 and MFN2 form complexes that regulate mitochondrial fusion and are essential for embryonic development.
When ectopically expressed, MFN1 inhibits the apoptosis-associated conformational change in Bax but not its mitochondrial translocation, indicating MFN1's role in regulating Bax activation on the outer mitochondrial membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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