Thermo Fisher Scientific SP1 Recombinant Rabbit Monoclonal Antibody (JF0950)
SP1 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, ChIP 등에 적합합니다. 높은 특이성과 민감도, 로트 간 일관성, 동물 유래 성분이 없는 제형을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ChIP assay (ChIP) | 0.5–2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0950 |
| Immunogen | Synthetic peptide within Human SP1 aa 544–588 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809774 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses. SP1 activity is regulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation. It also binds the PDGFR-alpha G-box promoter and may modulate cellular responses to DNA damage. SP1 is implicated in chromatin remodeling and plays a role in recruiting SMARCA4/BRG1 on the c-FOS promoter, as well as in regulating FE65 gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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