Thermo Fisher Scientific PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody (PCNA, 694)
PCNA 단백질을 인식하는 Mouse Monoclonal 항체로, 세포 증식 마커 분석에 적합. Western Blot, IHC, ICC/IF, Flow Cytometry 등에 활용 가능. 높은 특이성과 재현성을 제공하며, 다양한 종에 반응. 연구용으로만 사용.
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- 5111-MSM2-P0
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- pk
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Thermo Fisher Scientific PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody (PCNA, 694)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCNA, 694 |
| Immunogen | Recombinant full-length human PCNA protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature (RT).
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, then cool at RT for 20 min.
- This antibody shows broad species reactivity.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways.
PCNA binds to CDK inhibitor p21, structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
It serves as a marker for proliferating cells and is useful for assessing tumor proliferation status.
Located in the nucleus, PCNA acts as a cofactor of DNA polymerase delta, functioning as a homotrimer to enhance leading strand synthesis during replication.
Upon DNA damage, PCNA becomes ubiquitinated and participates in the RAD6-dependent DNA repair pathway.
Two transcript variants encode the same protein, and pseudogenes have been described on chromosome 4 and the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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