Thermo Fisher Scientific PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody (PCNA, 694)
PCNA 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, 인간 및 개 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능하며, 단백질 A/G로 정제된 액상 형태로 제공됩니다. 세포 증식 및 DNA 복제 관련 연구에 적합합니다.
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Thermo Fisher Scientific PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody (PCNA, 694)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCNA, 694 |
| Immunogen | Recombinant full length human PCNA protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
- This antibody shows broad species reactivity.
Target Information
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways.
It binds to CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and tumor prognosis, being expressed in early G1 and S phases.
Located in the nucleus, PCNA acts as a cofactor of DNA polymerase delta, forming a homotrimer to enhance leading strand synthesis.
Upon DNA damage, PCNA is ubiquitinated and participates in the RAD6-dependent repair pathway.
Two transcript variants encoding the same protein have been identified, with pseudogenes on chromosome 4 and X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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