Thermo Fisher Scientific Bovine IFN-gamma Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA (ELISA): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent

Product Specifications

Product Specific Information

Mature bovine IFNγ was expressed in Pichia pastoris X-33.
GenBank® Accession # AAB64477, amino acids 24–166.
N-terminal sequencing confirmed the first 10 amino acids as QGQFFREIEN.
Purity is >95% by SDS-PAGE analysis.

Activity:
The biological activity of recombinant bovine IFNγ was determined in a viral challenge assay using VS virus on MDBK cells. Plates were stained with crystal violet, visual CPE determination was performed, and absorbance was measured after dye solubilization.

Uses:
For in vitro applications. A dose-response assay is recommended to determine optimal concentration for specific experiments.

Reconstitution:
Reconstitute lyophilized material with 0.5 mL of distilled or deionized water.
Dilute in a buffer containing carrier protein, aliquot, and store at ≤ -70°C.
Avoid multiple freeze-thaw cycles.
PBS with 1% BSA or neutral media containing ≥10% FCS are suitable buffers.

Target Information

Interferon gamma (IFNγ, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits, showing species-specific biological activity.

Functions include:

• Antiviral and antitumor activity
• Induction of MHC class I and II molecules
• Macrophage activation
• Enhancement of immunoglobulin secretion by B lymphocytes
• Regulation of cytokine signaling via JAK-STAT pathway

Human IFNγ shows ~40% sequence homology with mouse IFNγ and no homology with IFNα or IFNβ.
Expression is upregulated by IL-2, FGF-basic, and EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFNγ gene are associated with aplastic anemia.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.