Thermo Fisher Scientific Bovine IFN-gamma Recombinant Protein
소에서 유래한 IFN-γ 재조합 단백질로, Pichia pastoris 발현 시스템을 통해 생산되었습니다. >95% 순도로 정제되었으며, 항바이러스 활성 및 면역 활성 연구용으로 적합합니다. ELISA, Western blot 등 다양한 in vitro 실험에 활용 가능합니다.
- 카탈로그번호
- RBOIFNGI
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Applications
- Western Blot (WB): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Bovine |
| Expression System | Pichia pastoris |
| Amino Acid Sequence | Bovine IFNγ, amino acids 24–166 (Accession # AAB64477) |
| Molecular Weight | 16.9 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE analysis |
| Activity | Determined in viral challenge assay using VSV on MDBK cells; 6 ng/mL causes 25% cytoprotection |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Mature bovine IFNγ was expressed in Pichia pastoris X-33.
GenBank® Accession # AAB64477, amino acids 24–166.
N-terminal sequencing confirmed the first 10 amino acids as QGQFFREIEN.
Purity is >95% by SDS-PAGE analysis.
Activity:
The biological activity of recombinant bovine IFNγ was determined in a viral challenge assay using VS virus on MDBK cells. Plates were stained with crystal violet, visual CPE determination was performed, and absorbance was measured after dye solubilization.
Uses:
For in vitro applications. A dose-response assay is recommended to determine optimal concentration for specific experiments.
Reconstitution:
Reconstitute lyophilized material with 0.5 mL of distilled or deionized water.
Dilute in a buffer containing carrier protein, aliquot, and store at ≤ -70°C.
Avoid multiple freeze-thaw cycles.
PBS with 1% BSA or neutral media containing ≥10% FCS are suitable buffers.
Target Information
Interferon gamma (IFNγ, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and cytokines.
It is a dimeric glycoprotein (~45 kDa) consisting of two 146 amino acid subunits, showing species-specific biological activity.
Functions include:
- Antiviral and antitumor activity
- Induction of MHC class I and II molecules
- Macrophage activation
- Enhancement of immunoglobulin secretion by B lymphocytes
- Regulation of cytokine signaling via JAK-STAT pathway
Human IFNγ shows ~40% sequence homology with mouse IFNγ and no homology with IFNα or IFNβ.
Expression is upregulated by IL-2, FGF-basic, and EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFNγ gene are associated with aplastic anemia.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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