Thermo Fisher Scientific Bovine IL-4 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species

Bovine

Expression System

P. pastoris

Amino acid sequence

aa 25-135

Molecular weight

12.6 kDa

Class

Recombinant

Type

Protein

Activity

ED50 = 0.44 - 0.67 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

For dilution, use a buffer containing carrier protein, e.g. PBS with 1% BSA or a neutral media containing 10% FCS and freeze in aliquots at -70°C.

Activity: The biological activity of recombinant bovine IL-4 was determined by measuring the proliferation of the factor-dependent human T-helper cell line, TF-1 (Kitamura, T. et al. (1989) J. Cell Physiol. 140:323). The ED50 (or 1 unit of activity) for this effect in our laboratories typically falls into the range of 0.44 - 0.67 ng/mL.

Expression: Mature bovine IL-4 was expressed in Pichia pastoris, X-33. GenBank® Accession # P30367, amino acids 25 - 135. For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: HKCDITLAEI.

Reconstitute with 0.5 mL of distilled water.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.