Thermo Fisher Scientific ZEB1 Monoclonal Antibody (OTI8E2), TrueMAB
인간 ZEB1 단백질을 인식하는 Mouse IgG2a 단클론 항체로 Western blot 및 IHC(P) 실험에 적합함. 무보존제, 동결건조 형태이며 PBS/trehalose 완충액에 용해. 연구용으로 EMT 및 전사 억제 관련 연구에 활용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8E2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–350 of human ZEB1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor and inhibits IL-2 gene expression. It modulates ATP1A1 promoter activity depending on cDNA quantity and cell type. ZEB1 represses E-cadherin promoters, inducing epithelial–mesenchymal transition (EMT) via SMARCA4/BRG1 recruitment. It also represses BCL6 transcription with CTBP1, positively regulates neuronal differentiation, and represses RCOR1 activation during neurogenesis. Mutations in ZEB1 may cause corneal dystrophy (posterior polymorphous and Fuchs endothelial).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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