Thermo Fisher Scientific ZEB1 Monoclonal Antibody (OTI9A3), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor and inhibits IL-2 gene expression. It modulates promoter activity of the ATP1A1 gene depending on cDNA quantity and cell type.
ZEB1 represses E-cadherin promoters, inducing epithelial-mesenchymal transition (EMT) via SMARCA4/BRG1 recruitment. It also represses BCL6 transcription with CTBP1 corepressor, positively regulates neuronal differentiation, and represses RCOR1 activation during neurogenesis.
Mutations in ZEB1 may cause corneal dystrophy (posterior polymorphous and Fuchs endothelial type).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.