Thermo Fisher Scientific Phospho-Tau (Ser202, Thr205) Monoclonal Antibody (AT8), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 106 publications 106 publications

Immunohistochemistry (IHC)

-

View 253 publications 253 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 24 publications 24 publications

ELISA (ELISA)

Assay-dependent

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cat, Chicken, Ferret, Fruit fly, Hamster, Horse, Human, Mammal, Mouse, Non-human primate, Rabbit, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AT8

Immunogen

Partially purified human PHF-Tau (aa 202, 205). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tau,uniProtId:P10636-1,ncbiNodeId:9606,antigenRange:202|205,antigenLength:758,antigenImageFileName:MN1020B_Tau_P10636-1_House_mouse.svg,antigenImageFileNamePDP:MN1020B_Tau_P10636-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223648

Product Specific Information

MN1020B targets PHF-tau (Ser202/Thr205) in IF, ELISA, and IHC (P) applications and shows reactivity with Human samples.

The MN1020B immunogen is partially purified human PHF-Tau.

MN1020B detects PHF-tau (Ser202/Thr205)a which has a predicted molecular weight of approximately 79 kDa.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.