Thermo Fisher Scientific Phospho-Tau (Thr181) Monoclonal Antibody (AT270)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

MN1050 recognizes PHF-Tau, tangles, and neurofilaments. It cross-reacts weakly with normal Tau and does not cross-react with recombinant unphosphorylated Tau. The epitope corresponds to the phosphorylated Thr181 residue (numbering according to human Tau40).

MN1050 detects PHF-Tau (Thr181), with a predicted molecular weight of approximately 79 kDa.
Purity is >95% as determined by SDS-PAGE.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, functioning as a linker between both structures.

Axonal polarity is determined by TAU/MAPT localization within the neuronal cell body domain defined by the centrosome. Short isoforms contribute to cytoskeletal plasticity, whereas longer isoforms stabilize the structure.

In its hyper-phosphorylated form, Tau forms paired helical filaments (PHF), the primary component of neurofibrillary tangles in Alzheimer’s disease (AD) brains. Hyper-phosphorylation disrupts Tau’s microtubule-binding ability, leading to microtubule destabilization and neuronal degeneration in AD.

Tau phosphorylation is mediated by several kinases, including GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is also found in neurofibrillary lesions of other CNS disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.