Thermo Fisher Scientific Phospho-Tau (Thr181) Monoclonal Antibody (AT270)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 83 publications 83 publications

Immunohistochemistry (IHC)

-

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-5 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

2-10 µg/mL

View 5 publications 5 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Functional Assay (Functional)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Chicken, Fruit fly, Hamster, Human, Mouse, Non-human primate, Rat, Yeast

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AT270

Immunogen

Partially purified human PHF-Tau if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tau,uniProtId:P10636-1,ncbiNodeId:9606,antigenRange:1-758,antigenLength:758,antigenImageFileName:MN1050_Tau_P10636-1_House_mouse.svg,antigenImageFileNamePDP:MN1050_Tau_P10636-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223651

Product Specific Information

MN1050 recognizes PHF-Tau, tangles and neurofilaments. Cross reacts weakly with normal Tau. Does not cross react with recombinant unphosphorylated Tau. The epitope of this antibody is the phosphorylated Thr181 residue (numbering according to human Tau40).

MN1050 detects PHF-tau (Thr181) which has a predicted molecular weight of approximately 79 kDa.

Purity is >95% as determined by SDS-PAGE.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.