Thermo Fisher Scientific Phospho-Tau (Thr212, Ser214) Monoclonal Antibody (AT100)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 59 publications 59 publications

Immunohistochemistry (IHC)

-

View 53 publications 53 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-5 µg/mL

View 11 publications 11 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 14 publications 14 publications

ELISA (ELISA)

5-10 µg/mL

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Chicken, Fruit fly, Hamster, Human, Mammal, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AT100

Immunogen

Purified human Tau if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tau,uniProtId:P10636-1,ncbiNodeId:9606,antigenRange:1-758,antigenLength:758,antigenImageFileName:MN1060_Tau_P10636-1_House_mouse.svg,antigenImageFileNamePDP:MN1060_Tau_P10636-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223652

Product Specific Information

MN1060 recognized PHF-Tau. No cross reactivity with normal biopsy-or-autopsy-derived Tau or fetal Tau has been observed. This suggests that this antibody detects an abnormal phosphorylation site unique to PHF-Tau. The epitope of this antibody has been show to contain phosphorylated Ser214 and phosphorylated Thr212 residues (numbering according to human Tau40).

MN1060 detects PHF-tau (Thr212/Ser214) which has a predicted molecular weight of approximately 79 kDa.

This product is a Low Endotoxin formulation.

Purity is >95% as determined by SDS-PAGE.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.