Thermo Fisher Scientific TOP1 Recombinant Rabbit Monoclonal Antibody (SC69-03)
TOP1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, ChIP 등 다양한 분석에 사용 가능. 높은 특이성과 민감도, 로트 간 일관성, 동물유래 성분 없는 제형 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:20,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC69-03 |
| Immunogen | Synthetic peptide within Human TOP1 aa 1–49 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809514 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
The TOP1 gene encodes a DNA topoisomerase enzyme that controls and alters the topologic states of DNA during transcription. It catalyzes transient breaking and rejoining of a single DNA strand to allow strand passage and alter DNA topology.
This gene is localized to chromosome 20 and has pseudogenes on chromosomes 1 and 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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