Thermo Fisher Scientific CD13 Recombinant Rabbit Monoclonal Antibody (SC70-01)
Western blot, IHC, ICC 등 다양한 응용에 적합한 재조합 토끼 단클론 항체. 인간, 마우스, 랫트 반응성. 높은 특이성과 재현성을 제공하며, Protein A 정제 및 동물 유래 성분 무첨가 포뮬레이션. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC70-01 |
| Immunogen | Synthetic peptide within Human CD13 aa 409–442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809512 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
CD13 (ANPEP) is a 150–170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. It functions as a metalloprotease that removes neutral amino acids from small peptides, thereby activating or inactivating bioactive peptides.
CD13 plays roles in:
- Extracellular matrix degradation
- Antigen processing and signal transduction
- Inflammatory response regulation
- Intercellular contact, cell motility, and vascularization
It is expressed by granulocytes, monocytes, and myeloid leukemia cells, and is associated with protection of leukemic cells against apoptosis and poor prognosis in carcinomas. CD13 also serves as an alpha receptor for coronavirus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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