Thermo Fisher Scientific CD21 Recombinant Rabbit Monoclonal Antibody (SC0681)
Thermo Fisher Scientific의 CD21 Recombinant Rabbit Monoclonal Antibody (SC0681)은 인간 및 생쥐 시료에 반응하며, 높은 특이성과 민감도를 제공합니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합합니다. 단백질 A 정제, 액상 형태로 안정적 저장이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:800 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC0681 |
| Immunogen | Synthetic peptide within Human CD21 aa 989–1033 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809513 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, particularly breakdown fragments attached to antigen surfaces.
Functions and characteristics:
- Involved in uptake and retention of immunocomplexes
- Supports survival of memory B cells
- Plays a role in humoral response to T-dependent antigens
- Serves as Epstein-Barr virus receptor
- Participates in prion targeting and neuroinvasion
- Exists in soluble form (sCD21) retaining ligand-binding ability
- Associates with CD19, CD81, and Leu13 in B cell activation complexes
- Genetic variations linked to systemic lupus erythematosus type 9 (SLEB9)
- Alternative splicing produces different isoforms
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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