Thermo Fisher Scientific RAPGEF1 Monoclonal Antibody (OTI2F5), TrueMAB
RAPGEF1 단백질을 인식하는 고특이성 Monoclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용 가능. Mouse IgG1 형식이며, Human, Mouse, Rat, Dog 반응성. 고순도 Affinity chromatography 정제, Lyophilized 형태로 안정적 보관 가능.
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Applications 및 Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F5 |
| Immunogen | Full length human recombinant protein of human RAPGEF1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
This gene encodes a human guanine nucleotide exchange factor that transduces signals from CRK by binding to the SH3 domain of CRK and activating several Ras family GTPases.
This signaling cascade may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation.
Several alternatively spliced transcript variants have been described, though the full-length nature of some variants remains undetermined.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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