Thermo Fisher Scientific RAPGEF1 Monoclonal Antibody (OTI2B5), TrueMAB
RAPGEF1 단백질을 인식하는 마우스 단클론 항체로 Western blot과 IHC(P)에서 사용 가능. HEK293T 세포에서 발현된 full-length 인간 단백질을 면역원으로 제작. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 사용 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B5 |
| Immunogen | Full length human recombinant protein of human RAPGEF1 produced in HEK293T cell. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RAPGEF1 encodes a guanine nucleotide exchange factor that transduces signals from CRK by binding the SH3 domain of CRK and activating Ras family GTPases.
This signaling pathway may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation.
Several alternatively spliced transcript variants have been described, though the full-length nature of some remains undetermined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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