Thermo Fisher Scientific LSD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat, Zebrafish

Published species

Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptides corresponding to residues 100-150 of human LSD1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LSD1,uniProtId:O60341-1,ncbiNodeId:9606,antigenRange:100-150,antigenLength:852,antigenImageFileName:PA1-41697_LSD1_O60341-1_Rabbit.svg,antigenImageFileNamePDP:PA1-41697_LSD1_O60341-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 0.2% gelatin

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1070348

Product Specific Information

Suggested positive control: brain lysate, human brain protein.

Target Information

LSD1 is a histone demethylase that specifically demethylates Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys-4 of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys-4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate Lys-9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.