Thermo Fisher Scientific Bcl-rambo Polyclonal Antibody
Rabbit polyclonal antibody against human Bcl-rambo. Validated for Western blot applications. Useful for studying apoptosis and Bcl-2 family protein regulation. Supplied as liquid, unconjugated form. Recommended storage at 4°C short term, -20°C long term.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a portion of human Bcl-rambo |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Conditions | Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2065330 |
Product Specific Information
Suggested positive control: HeLa whole cell extract or HeLa cell lysate.
Target Information
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in various diseases such as cancer. Members of the Bcl-2 family are critical regulators of apoptosis, characterized by conserved Bcl-2 homology (BH) domains.
Bcl-rambo, a novel and widely expressed member, localizes to mitochondria and induces apoptosis when overexpressed. This effect can be inhibited by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2.
Bcl-rambo shares homology with anti-apoptotic Bcl-2 family members but has a unique 250 amino acid insertion preceding its C-terminal membrane anchor. This region alone can induce apoptosis more efficiently than the BH domains, suggesting Bcl-rambo's role in additional pro-apoptotic pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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