Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (M20)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M20

Immunogen

Keratin isolated from the human breast carcinoma cell line MCF-7. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 8,uniProtId:P05787-1,ncbiNodeId:9606,antigenRange:1-483,antigenLength:483,antigenImageFileName:MA1-06318_Cytokeratin_8_P05787-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06318_Cytokeratin_8_P05787-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559794

Product Specific Information

MA1-06318 detects cytokeratin 8 in rat, human, and rabbit samples.

MA1-06318 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry and immunohistochemistry (frozen, paraffin).

The MA1-06318 immunogen is keratin isolated from the human breast carcinoma cell line MCF-7.

Store at 4ºC or in small aliquots at -20ºC.

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.