Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (M20)
Cytokeratin 8 단백질을 인식하는 Mouse monoclonal antibody (Clone M20). Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. Human, Rat, Rabbit 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C 또는 -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:1,000 | View 6 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:200 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:200 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | View 1 publication |
| Flow Cytometry (Flow) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rabbit, Rat |
| Published species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M20 |
| Immunogen | Keratin isolated from the human breast carcinoma cell line MCF-7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_559794 |
Product Specific Information
MA1-06318 detects cytokeratin 8 in rat, human, and rabbit samples.
It has been successfully used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry (frozen and paraffin).
The immunogen is keratin isolated from the human breast carcinoma cell line MCF-7.
Store at 4ºC or in small aliquots at -20ºC.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12.
Type I and type II keratins heteropolymerize to form intermediate-sized filaments in epithelial cells.
Cytokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple epithelial cells.
This protein plays a key role in maintaining cellular structural integrity and participates in signal transduction and differentiation.
Mutations in this gene are associated with cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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