Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (RGE53)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:200

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Flow Cytometry (Flow)

Assay-dependent

-

In vitro Assay (IV)

-

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Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mouse, Pig, Rabbit, Rat, Zebrafish

Published species

Mouse, Rat, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RGE53

Immunogen

Cytoskeletal preparation of HeLa cells if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 18,uniProtId:P05783-1,ncbiNodeId:9606,antigenRange:1-430,antigenLength:430,antigenImageFileName:MA1-06326_Cytokeratin_18_P05783-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06326_Cytokeratin_18_P05783-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559772

Product Specific Information

MA1-06326 detects cytokeratin 18 in human, mouse, rat, hamster, rabbit, chicken, canine, zebrafish and swine samples.

MA1-06326 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry.

The MA1-06326 immunogen is a cytoskeletal preparation from cells.

Store at 4ºC or in small aliquots at -20ºC.

Target Information

Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. Further, cytokeratin 18 is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for the cytokeratin 18 gene. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.