Thermo Fisher Scientific Cholesterol Oxidase Polyclonal Antibody, HRP
Western blot, ELISA, IP에 적합한 HRP 결합형 Polyclonal 항체로, Streptomyces 유래 Cholesterol Oxidase를 인식합니다. 염 교환 크로마토그래피로 정제되었으며, 안정적인 보관 및 다양한 연구용 응용에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cholesterol Oxidase [Streptomyces] |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15 M NaCl |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Cholesterol Oxidase antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis against the specified buffer.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, as well as purified and partially purified Cholesterol Oxidase [Microorganism].
Cross-reactivity against Cholesterol Oxidase from other sources is unknown.
Target Information
Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This protein is involved in the cholesterol metabolism pathway, which is part of steroid metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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