Thermo Fisher Scientific Sucrose Phosphorylase Polyclonal Antibody, HRP

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:5,000

ELISA

• Tested Dilution: 1:5,000–1:20,000

Immunoprecipitation (IP)

• Tested Dilution: 1:500–1:5,000

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot contents and freeze at -20°C or below.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as undiluted liquid.
• Dilute only prior to immediate use.

Sucrose Phosphorylase antibody is an IgG fraction purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified Sucrose Phosphorylase (E.coli).
Cross-reactivity against Sucrose Phosphorylase from other sources is unknown.

Target Information

Sucrose phosphorylase catalyzes the reaction:
Sucrose + phosphate → D-fructose + α-D-glucose 1-phosphate

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.