Thermo Fisher Scientific Pyranose Oxidase Polyclonal Antibody, HRP

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Pyranose Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process including delipidation, salt fractionation, and ion-exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, as well as purified and partially purified Pyranose Oxidase [E.coli].
Cross-reactivity against Pyranose Oxidase from other sources is unknown.

Target Information

Pyranose Oxidase catalyzes the oxidation of various aldopyranoses and disaccharides on carbon-2 to the corresponding 2-keto sugars, concomitant with the reduction of O₂ to H₂O₂.
It plays an important role in lignin degradation of wood rot fungi by supplying the essential cosubstrate H₂O₂ for ligninolytic peroxidases such as lignin peroxidase and manganese-dependent peroxidase.
The preferred substrate is D-glucose, which is converted to 2-dehydro-D-glucose. It also acts on D-xylose, L-sorbose, D-galactose, and 1,5-anhydroglucitol, a diagnostic marker of diabetes mellitus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.