Thermo Fisher Scientific VIM Monoclonal Antibody (OTI6B12), TrueMAB
Vimentin 단백질을 인식하는 Mouse monoclonal antibody (OTI6B12). Western blot에 적합하며, 인간 시료에 반응. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도. 연구용으로 세포 내 단백질 수송 및 발달 관련 연구에 활용 가능.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6B12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210–466 of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane.
During early cellular development, vimentin is co-expressed with desmin and later replaced by tissue-specific IFPs.
Vimentin plays a role in steroid synthesis by managing cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to cytoskeletal reorganization, promoting cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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