Thermo Fisher Scientific VIM Monoclonal Antibody (OTI7B10), TrueMAB
Vimentin 단백질을 인식하는 Mouse Monoclonal Antibody로, Western Blot에 최적화되어 있습니다. E.coli에서 생산된 full-length human recombinant protein을 면역원으로 사용합니다. Lyophilized 형태이며, PBS buffer에 trehalose가 포함되어 안정성이 우수합니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7B10 |
| Immunogen | Full length human recombinant protein of human VIM produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final concentration of ~1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin is co-expressed with desmin during early cellular development and later replaced by tissue-specific intermediate filament proteins.
It is also implicated in steroid synthesis regulation through its role as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin leads to cytoskeletal reorganization that facilitates cholesterol transport to mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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