Thermo Fisher Scientific VIM Monoclonal Antibody (OTI1F10), TrueMAB
Human Vimentin 단백질을 인식하는 Mouse IgG2a 단클론 항체로, Western blot에 최적화되어 있습니다. Lyophilized 형태이며 PBS buffer와 trehalose 포함. 연구용으로 세포 내 단백질 수송 및 중간 필라멘트 연구에 적합.
- 판매단위
- pk
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Applications
- Western Blot (WB)
Tested Dilution
- 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F10 |
| Immunogen | Full length human recombinant protein of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin and desmin are expressed during early cellular development and later replaced by tissue-specific IFPs.
Vimentin is involved in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation by protein kinase leads to filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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