Thermo Fisher Scientific VIM Monoclonal Antibody (OTI1F10), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Vimentin is a developmentally regulated intermediate filament protein found in cells of mesenchymal origin. It plays a role in intracellular protein transport between the nucleus and plasma membrane. Vimentin and desmin are expressed during early cellular development and later replaced by tissue-specific IFPs.
Vimentin is involved in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation by protein kinase leads to filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroidogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.