Thermo Fisher Scientific Phospho-EIF2S1 (Tyr150) Polyclonal Antibody
EIF2S1 단백질의 Tyr150 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 사용 가능. 인산화된 eIF2α의 내인성 수준을 특이적으로 검출하며, Human, Mouse, Rat 시료에 반응. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human EIF2S1 (Accession P05198), corresponding to amino acid residues around phosphorylated Tyr150. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816888 |
Product Specific Information
Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Tyr150.
Target Information
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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