Thermo Fisher Scientific Phospho-p53 (Ser99) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody detects endogenous levels of p53 only when phosphorylated at Ser99.

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation. p53 upregulates transcription of genes such as p21waf1 involved in G1 or G2/M arrest. Structurally, p53 includes:

• N-terminal transactivation domain
• Central DNA-binding domain
• Oligomerisation domain
• C-terminal regulatory domain

Phosphorylation at Ser15 is critical for activation and stabilization, while Ser392 phosphorylation is associated with tumor formation. p53 is expressed at low levels in normal cells but elevated in transformed cell lines, contributing to malignancy. Mutations in TP53 can lead to loss of tumor suppressor function and are implicated in various cancers, including Li-Fraumeni syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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