Thermo Fisher Scientific DYKDDDDK Tag Recombinant Mouse Monoclonal Antibody (M2.1)
DYKDDDDK(FALG) 태그를 특이적으로 인식하는 재조합 마우스 모노클로날 항체. Western blot, ICC/IF, ELISA 등 다양한 분석에 사용 가능. HEK293 발현 시스템 기반, Protein A로 정제된 고순도 항체. 연구용 단백질 검출 및 정제에 최적화.
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Applications
- Western Blot (WB): Assay-dependent
- Immunocytochemistry (ICC/IF): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | M2.1 |
| Immunogen | Interleukin 2 genetically fused to the peptide DYKDDDDK, corresponding to the DDDDK epitope tag |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3074506 |
Product Specific Information
Specificity: This antibody recognizes the DYKDDDDK-tag, a widely used epitope tag.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used as a protein tag to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys.
It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research for protein-protein interaction studies, expression analysis, and localization studies.
The FLAG tag structure is optimized for compatibility with attached proteins, being more hydrophilic and less likely to denature or inactivate them.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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