Thermo Fisher Scientific DYKDDDDK Tag Chimeric Recombinant Rabbit Monoclonal Antibody (M2.1)
DYKDDDDK(FALG) 태그를 인식하는 Thermo Fisher Scientific의 재조합 토끼 단일클론 항체. WB, ICC/IF, ELISA 등 다양한 분석에 사용 가능. 높은 특이성과 재현성 제공. 연구용 단백질 검출 및 정제에 적합.
- 카탈로그번호
- MA548064
- 판매단위
- pk
카탈로그
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 0.0169 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | M2.1 |
| Immunogen | Interleukin 2 genetically fused to the peptide DYKDDDDK, corresponding to the DDDDK epitope tag |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3074507 |
Product Specific Information
This antibody recognizes the DYKDDDDK-tag, a widely used epitope tag.
Target Information
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence commonly used to facilitate protein purification, detection, and localization.
The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is typically fused to the N- or C-terminus of a protein of interest, allowing easy identification and isolation using anti-FLAG antibodies.
The DYKDDDDK tag is widely used in molecular biology and biochemistry research, including protein-protein interaction studies, protein expression analysis, and protein localization.
The FLAG tag structure is optimized for compatibility with attached proteins, being more hydrophilic than other common epitope tags, and therefore less likely to denature or inactivate proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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