Thermo Fisher Scientific Human IL-1 alpha Recombinant Protein
인간 IL-1α 재조합 단백질로, 염증성 사이토카인 연구에 사용됩니다. E. coli 발현 시스템에서 생산되며, >95% 순도와 낮은 엔도톡신 수준을 가집니다. 세포 증식 기반 기능 분석에 적합하며, 캐리어 프리 형태로 제공됩니다.
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- PHC001x (5개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
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5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human IL-1 alpha Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA |
| Molecular Weight | 18 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 0.001 - 0.005 ng/mL; determined by dose-dependent proliferation of mouse D10S cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge vial before opening. Reconstitute lyophilized human IL-1α in deionized water to 0.1–1.0 mg/mL for full activity.
Stock solutions should be divided into aliquots and stored at ≤ -20°C.
Further dilutions should be made in low endotoxin medium or buffered solution containing heat-inactivated FCS or tissue culture grade BSA.Storage:
Lyophilized form: store at 2°C–8°C, preferably desiccated.
Reconstituted form: store at ≤ -20°C (avoid frost-free freezer).
Minimize freeze-thaw cycles.
Target Information
IL-1α (Interleukin-1 alpha) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells.
Encoded by the IL1A gene on chromosome 2q13, it signals through IL-1RI and IL-1RII receptors shared with IL-1β.
IL-1α plays a key role in innate host defense by inducing other proinflammatory cytokines and acute-phase responses.
Its activity is regulated by IL-1 Receptor Antagonist (IL-1RA), which competes for receptor binding.
It is involved in immune responses, inflammation, and hematopoiesis, and can induce apoptosis upon cell injury.
IL-1α influences NF-κB and MAP kinase pathways, stimulates IL6 and COX-2 (PTGS2) expression, and enhances nitric oxide production.
High IL-1α levels are linked to chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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