
Thermo Fisher Scientific Human IL-3 Recombinant Protein
인간 IL-3 재조합 단백질로, 세포 성장 및 분화 연구에 활용 가능. E. coli 발현 시스템에서 제조되었으며 순도 95% 이상. 기능적 활성 검증 완료, 엔도톡신 농도 0.1 ng/µg 미만. 동결건조 형태로 제공되며 연구용으로만 사용.
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Thermo Fisher Scientific Human IL-3 Recombinant Protein
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | APMTQTTPLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF |
| Molecular Weight | 15 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 = 0.1 - 0.25 ng/mL (dose-dependent proliferation of human TF-1 cells) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution: Briefly centrifuge vial before opening. Reconstitute lyophilized recombinant human IL-3 in sterile distilled water to 0.1–1.0 mg/mL. Divide into aliquots and store at ≤ -20°C. Further dilution should be done in medium or buffer containing carrier protein (e.g., PBS with 0.1% BSA).
Storage: Lyophilized recombinant human IL-3 should be stored at 2–8°C, preferably desiccated. Store reconstituted protein at ≤ -20°C (avoid frost-free freezer). Minimize freeze-thaw cycles.
Target Information
Interleukin 3 (IL-3), also known as Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells, and eosinophils.
IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages. Its functions are often enhanced by co-stimulation with other cytokines.
Human and mouse IL-3 share only 29% amino acid sequence identity, and IL-3 bioactivity is species-specific—human IL-3 does not act on mouse cells.
IL-3 supports proliferation, differentiation, and apoptosis of hematopoietic cells, and may have neurotrophic activity linked to neurological disorders.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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