Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Alexa Fluor 561, eBioscience
인간 CD127(IL-7Rα)을 인식하는 eBioRDR5 클론의 Mouse IgG1 단일클론 항체로, Alexa Fluor 561 형광체 결합. Flow Cytometry에 최적화되어 있으며, T세포 및 Treg 분석에 유용. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), Alexa Fluor 561, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 561 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioRDR5 |
| Conjugate | Alexa Fluor™ 561 |
| Excitation/Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896211 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 647, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Yellow Series
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue / Red / UV Series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 forms a complex with CD132 (common gamma chain) to create the IL-7 receptor (IL-7R). CD127 is a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.
IL-7 binding triggers signal transduction via tyrosine kinase pathways including Jak/STAT. IL-7 is essential for lymphocyte development and peripheral T-cell homeostasis. CD127 expression is notably down-regulated on CD4+CD25+ regulatory T cells (Tregs), making CD127 a marker for enriched Treg populations expressing Foxp3.
Binding of eBioRDR5 antibody to PBMCs is blocked by pre-incubation with recombinant human IL-7.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells; recommended 5 µL (0.25 µg) per test (100 µL final volume).
Emission: 575 nm (Yellow-Green Laser compatible)
Target Information
CD127 (IL-7Rα) is a glycoprotein regulating lymphopoiesis. The active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain activates signal transduction, while the alpha chain mediates specific signaling. CD127 promotes proliferation of precursor B cells, thymocytes, and mature T cells. Dysfunction is associated with severe combined immunodeficiency (SCID).
For Research Use Only. Not for use in diagnostic procedures.
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