Thermo Fisher Scientific Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eFluor 660, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 600
• Custom conjugation available

Product Specific Information

The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein expressed in kidney podocytes, epithelial, and mesothelial cells. It serves as a potent marker for lymphatic endothelium and is also expressed in thymic subcapsular epithelial cells. Podoplanin-deficient mice exhibit respiratory defects and congenital lymphedema due to lymphatic patterning failure.

Applications Reported: Flow cytometric analysis.
Applications Tested: Validated for flow cytometric analysis using TE-71 cell line at ≤0.125 µg/test (100 µL final volume). Optimal titration recommended.

eFluor™ 660 replaces Alexa Fluor™ 647, emitting at 659 nm and excited by the red laser (633 nm).
Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin-type 1 glycoprotein (40–43 kDa) expressed in lymphatic endothelial and follicular dendritic cells. It localizes in stromal cells of lymphoid tissues and thymic epithelial cells. Podoplanin acts as a ligand for CLEC-2 and is associated with tumor invasiveness and metastasis. It also participates in cell migration and lymphatic endothelial regulation. Expression is induced by Src activation of Cas and is observed in Th17 cells. Dysfunction is linked to lymphangioma and subglottic neoplasm, and it serves as a marker for lung injury.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지