Thermo Fisher Scientific IFN gamma Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: Assay-dependent

ELISA (ELISA)

• Tested Dilution: Assay-dependent

Functional Assay

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• In Western blot, this antibody detects a band at approximately 18 kDa.
• Rabbit anti-Chicken interferon gamma antibody recognizes chicken interferon gamma (chIFN-gamma).

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin, and other cytokines.
It is a dimeric glycoprotein consisting of two 146 amino acid subunits (~45 kDa homodimer). On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.

The biological activity of IFN gamma is highly species-specific — human IFN gamma does not cross-react with mouse IFN gamma.
Functions include:

• Antiviral activity
• Tumor antiproliferative activity
• Induction of class I and II MHC
• Macrophage activation
• Enhanced immunoglobulin secretion by B lymphocytes

IFN gamma is involved in cytokine regulation and acts synergistically with other cytokines. Activation occurs via IFN gamma receptor I and II, triggering the JAK-STAT pathway.
Human IFN gamma shares about 40% sequence homology with mouse IFN gamma. Expression is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.