Thermo Fisher Scientific Phospho-MEK1 (Thr386) Recombinant Rabbit Monoclonal Antibody (8H5L16)
Recombinant rabbit monoclonal antibody specific for phospho-MEK1 (Thr386). Validated for WB and ICC/IF in human and mouse samples. Offers high specificity, lot-to-lot consistency, and animal origin-free formulation. Suitable for research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H5L16 |
| Immunogen | Chemically synthesized phosphopeptide derived from a region of human MEK1 containing threonine 386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2688291 |
Product Specific Information
This antibody is predicted to react with Monkey, Rat, and Mouse.
Antigen is similar to product #44-462G. The sequence is conserved in many species including mouse, rat, chimp, hamster, and rabbit.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation.
MAP2K1 is activated by various growth factors, cytokines, membrane-dependent depolarization, and calcium influx.
MEK1 is activated by phosphorylation of serine 218 and 222 residues by Raf1. It is involved in signaling during stress response, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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