Thermo Fisher Scientific mGLuR2 Recombinant Rabbit Monoclonal Antibody (15H9L5)
mGluR2 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF에 사용 가능. Expi293 시스템에서 발현되었으며 높은 특이성과 재현성을 제공. Human 및 Rat 반응성, 단기 4°C 보관, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 15H9L5 |
| Immunogen | Peptide corresponding to human mGLUR2 [aa429–aa444] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2734818 |
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit, and Bovine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences are cloned from immunoreactive rabbits, and individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Glutamate is the main excitatory neurotransmitter in the brain. Traditionally, it was known to act on ligand-gated receptor channels such as NMDA, AMPA, and kainate receptors, which mediate fast excitatory synaptic transmission.
Recent findings show that glutamate also regulates enzymes producing second messengers via G-protein-coupled receptors known as metabotropic glutamate receptors (mGluRs).
- Group I receptors (mGluR1a, mGluR5): Stimulate phospholipase C, increasing phosphoinositide turnover and intracellular calcium release.
- Group II and III receptors (mGluR2, mGluR3): Inhibit adenylyl cyclase activity.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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