Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), PE, eBioscience
마우스 CD45.2 분자를 인식하는 단클론 항체로, PE 형광 표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 0.5 µg/test로 사용 가능합니다. CD45.2를 발현하는 다양한 마우스 균주 분석에 적합하며, 4°C 암소 보관이 필요합니다.
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- 12-0454-8x (3개 옵션)
- 판매단위
- pk
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3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | View 114 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 104 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465677 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605, 650, 711, 786
- eFluor 450, 506
- FITC
- NovaFluor Blue 510, 555, 585, 610-70S, 660-120S
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 600, 645, 780
- Request custom conjugation
Product Specific Information
Description:
The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. These include C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Titrate for optimal performance.
Excitation/Emission:
488–561 nm excitation; 578 nm emission.
Compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It plays a key role in lymphocyte development and antigen signaling through regulation of Src-family kinases.
Multiple isoforms arise from alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic regions. These isoforms vary in membrane localization and expression depending on cell type and physiological state.
CD45.2 also promotes cell survival via integrin-mediated signaling and is involved in DNA fragmentation during apoptosis. CD45RA is an isoform with restricted expression among lymphoid subtypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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