Thermo Fisher Scientific Human DKK-3 Recombinant Protein, PeproTech
Recombinant Human DKK-3 단백질로 CHO 세포에서 발현된 고순도(≥98%) 단백질입니다. WNT 신호 억제 연구에 활용되며, MC3T3 E1 세포의 알칼리성 인산분해효소 활성을 억제하는 기능을 가집니다. 분자량은 약 36.3 kDa이며, 동결건조 형태로 제공됩니다.
- 카탈로그번호
- 120-46-xxx (6개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
6개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human DKK-3 Recombinant Protein, PeproTech
Applications
- Functional Assay (Functional)
Tested Dilution: Assay-dependent
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | CHO cells |
| Amino Acid Sequence | APAPTATSAP VKPGPALSYP QEEATLNEMF REVEELMEDT QHKLRSAVEE MEAEEAAAKA SSEVNLANLP PSYHNETNTD TKVGNNTIHV HREIHKITNN QTGQMVFSET VITSVGDEEG RRSHECIIDE DCGPSMYCQF ASFQYTCQPC RGQRMLCTRD SECCGDQLCV WGHCTKMATR GSNGTICDNQ RDCQPGLCCA FQRGLLFPVC TPLPVEGELC HDPASRLLDL ITWELEPDGA LDRCPCASGL LCQPHSHSLV YVCKPTFVGS RDQDGEILLP REVPDEYEVG SFMEEVRQEL EDLERSLTEE MALREPAAAA AALLGGEEI |
| Molecular Weight | 36.3 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | <1 EU/µg |
| Activity | Inhibits alkaline phosphatase activity in differentiating MC3T3 E1 cells (ED50: 2.0–4.0 ng/ml) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human DKK-3 expressed in CHO cells is a glycoprotein with a calculated molecular weight of 36.3 kDa and 329 amino acid residues. Due to glycosylation, Human DKK-3 migrates at an apparent molecular weight of approximately 39–49 kDa by SDS-PAGE analysis under non-reducing conditions.
This product is shipped at ambient temperature. For storage, handling, and reconstitution information, please refer to the lot-specific Certificate of Analysis.
Target Information
DKK3, like DKK1, DKK2, and DKK4, has an N-terminal signal peptide and two conserved cysteine-rich domains separated by a linker region containing 10 cysteine residues each. The second cysteine region may have a lipid-binding function facilitating WNT/DKK interactions at the plasma membrane.
The linker region contains 50–55 amino acids in DKK1, DKK2, and DKK4, but only 12 amino acids in DKK3. All DKKs have potential cleavage sites for furin-type proteases.
DKK3 transcript shows high expression in heart, brain, and spinal cord (Northern blot analysis). In situ hybridization reveals strong expression in mouse brain, eye, and heart.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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