Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), eBioscience
Rat CD106(VCAM-1)을 인식하는 단클론 항체로, Flow Cytometry, WB, IHC(F), IP 등에 사용 가능. 0.5 µg/test 권장. 액상 형태, 0.5 mg/mL 농도, PBS(pH 7.2)에 0.09% sodium azide 포함. 4°C 보관, 높은 순도(>90%) 확보.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMR106 (MR106) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_529472 |
Additional Formats:
Product Specific Information
The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation.
CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), interactions that are important for early lymphocyte and myeloid development in bone marrow and thymus. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in inflammatory response.
Applications Reported:
This eBioMR106 (MR106) antibody has been reported for use in:
- Flow cytometric analysis
- Immunoprecipitation
- Immunoblotting (WB)
- Immunohistology staining of frozen tissue sections
Applications Tested:
Tested by flow cytometric analysis of rat bone marrow cells. Recommended usage ≤ 0.5 µg per test (defined as the amount of antibody staining a cell sample in a final 100 µL volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Antibody titration is recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, leukocyte-endothelial adhesion, and signal transduction, and may play a role in inflammatory diseases.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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