Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (STA), eBioscience
인간 CD106(VCAM-1)을 인식하는 STA 클론 단일클론 항체로, 염증 반응 관련 연구에 적합. Flow Cytometry, IHC(F), ICC/IF 등 다양한 응용 가능. 0.5 mg/mL 농도의 액상형 제품으로, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 11 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | STA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864966 |
Available Conjugated Formats
Product Specific Information
The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin α4β1 (VLA-4, CD49d/CD29) and Integrin α4β7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.
Applications Reported: STA has been reported for use in flow cytometric analysis and immunohistochemical staining of frozen tissue sections.
Applications Tested: The STA antibody has been tested by flow cytometric analysis of TNFα-activated HUVEC cells. It can be used at ≤1 µg per test (defined as the amount of antibody that stains a cell sample in 100 µL final volume). Optimal performance should be determined empirically.
Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, leukocyte-endothelial adhesion, and signal transduction, and may play a role in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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