Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), eFluor 450, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test
  View 7 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Brilliant UV 615
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on plasmacytoid dendritic cells (pDC) or type I IFN-producing cells (IPC) in naive mice.
Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and respond rapidly to viral infection.

Siglec-H is a transmembrane protein of the immunoglobulin superfamily that binds sialic acid but lacks a cytoplasmic ITIM domain. Instead, it associates with DAP12 to enable signal transduction.

The eBio440c antibody inhibits pDC function by reducing IFNα secretion in response to CpG stimulation. Some mouse strains (e.g., SJL) show higher pDC percentages than C57BL/6.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤0.5 µg/test, 100 µL final volume)
  Cell number should be optimized (10⁵–10⁸ cells/test).
• Fluorophore: eFluor® 450 (alternative to Pacific Blue®)
  • Excitation: 405 nm
  • Emission: 445 nm
  • Laser: Violet
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Siglec-H is expressed on plasmacytoid dendritic cells (pDC) or type I IFN-producing cells (IPC) in mice.
It binds sialic acid and interacts with DAP12 for signaling. The eBio440c clone inhibits pDC function by reducing IFNα secretion.
Strain-dependent variation in pDC frequency has been observed (e.g., SJL > C57BL/6).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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