Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available upon request

Product Specific Information

The WM59 monoclonal antibody reacts with human CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells and at low levels on leukocytes and platelets. CD31 mediates homotypic interactions important for adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test in a 100 µL final staining volume.

Fluorochrome Information:
eFluor™ 450 is an alternative to Pacific Blue™, excited at 405 nm and emitting at 445 nm (Violet laser). Ensure instrument compatibility.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular compartment, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.

CD31 plays roles in:

• Integrin-mediated adhesion
• Transendothelial migration
• Angiogenesis and apoptosis
• Negative regulation of immunoreceptor signaling
• Autoimmunity and macrophage phagocytosis
• IgE-mediated anaphylaxis and thrombosis

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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