Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience
CD31(PECAM-1)을 인식하는 WM-59 단클론 항체로, eFluor 450 형광으로 표지되어 있습니다. 인간 및 마우스 시료에 사용 가능하며, 주로 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 405/445 nm 파장에서 Violet 레이저로 여기됩니다. 연구용 전용 제품입니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), eFluor 450, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | - |
| Immunohistochemistry (IHC) | - | 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 6 publications |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | 38 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM-59 (WM59) |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_10853015 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available upon request
Product Specific Information
The WM59 monoclonal antibody reacts with human CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells and at low levels on leukocytes and platelets. CD31 mediates homotypic interactions important for adhesion, cell-cell and cell-matrix interactions, and signal transduction.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test in a 100 µL final staining volume.
Fluorochrome Information:
eFluor™ 450 is an alternative to Pacific Blue™, excited at 405 nm and emitting at 445 nm (Violet laser). Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular compartment, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays roles in:
- Integrin-mediated adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immunoreceptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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