Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 660, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 660

Applications

• Flow Cytometry (Flow): 5 µL (0.2 µg)/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, 532, 561, 647, 700, APC, APC-eFluor 780, Biotin, Brilliant UV/Violet/Yellow series, eFluor 450/506/660, FITC, PE, PE-Cyanine series, PerCP series, Super Bright series, NovaFluor Blue/Red/UV/Violet/Yellow series, and Functional Grade.
(Custom conjugation available upon request.)

Product Specific Information

Description:
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 forms a heterodimer with CD132 (common gamma chain) to create the IL-7 receptor complex, essential for lymphocyte development and T-cell homeostasis. CD127 expression is observed on immature B cells, thymocytes, and most mature T cells, with transient downregulation upon activation.

IL-7 binding triggers signal transduction via tyrosine kinase pathways, including Jak/STAT. CD127 is also involved in TCR locus rearrangement in γδ T cells.

CD127 expression is notably downregulated on CD4⁺CD25⁺ regulatory T cells (Tregs). Co-expression of CD4 and CD25 is commonly used to identify Tregs, but CD127 downregulation provides a more specific marker correlated with Foxp3 expression and suppressive activity.

Binding of this antibody to PBMCs can be blocked by pre-incubation with recombinant human IL-7 (Product #14-1079-80).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested for flow cytometry using normal human peripheral blood cells at 5 µL (0.2 µg) per test. Optimal cell number: 10⁵–10⁸ cells/test.

Note:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Product #C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before mixing with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Conjugates:
Based on Phiton™ technology utilizing engineered nucleic acid dye structures for optimized fluorescence with reduced spillover and spread.
Excitation: 637 nm; Emission: 659 nm; Laser: 633–640 nm (Red).

Target Information

CD127 (Interleukin-7 Receptor alpha, IL7Rα):
A glycoprotein regulating lymphopoiesis. The IL-7 receptor is an α/γ chain heterodimer; the γ(c) chain activates signaling, while the α chain mediates specific intracellular interactions. CD127 promotes proliferation of B cell precursors, thymocytes, and T cell subsets. Dysregulation is associated with severe combined immunodeficiency (SCID).

For Research Use Only. Not for diagnostic procedures or resale without authorization.

제품 이미지