Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Red 685

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor (488, 532, 561, 647, 700), APC, APC-eFluor 780, Biotin, Brilliant UV (496–805), Brilliant Violet (421–786), eFluor (450, 506, 660), FITC, PE, PE-Cyanine series, PerCP, Super Bright series, NovaFluor Blue/Red/Yellow/Violet series, and Functional Grade.
Custom conjugation available upon request.

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor (IL-7R), a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells. IL-7 binding activates signal transduction through Jak/STAT and other kinase pathways, essential for lymphocyte development and T-cell homeostasis.

CD127 expression is down-regulated on CD4⁺CD25⁺ regulatory T cells (Tregs). CD4⁺CD25⁺CD127⁻ cells are enriched for Foxp3⁺ Tregs with suppressive activity, providing a more accurate Treg identification marker.

Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).
Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for human peripheral blood cells at 5 µL/test (0.6 µg/test, 100 µL final volume, 10⁵–10⁸ cells/test)

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Use CellBlox Plus Blocking Buffer (Product #C001T06F01) for optimal staining. Add 5 µL per sample before combining with cells.
• For single-color controls: 5 µL per 100 µL cell suspension (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, engineered for precise fluorescence spectra with minimal spillover and spread.

• Excitation: 637 nm
• Emission: 685 nm
• Laser: 633–640 nm (Red)

Target Information

CD127 (Interleukin-7 Receptor alpha, IL7Rα) is a glycoprotein regulating lymphopoiesis. The IL7R complex (alpha/gamma heterodimer) mediates IL-7–induced signaling. The gamma(c) chain activates signal transduction, while the alpha chain determines specific pathways. CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature T cells. Dysregulation is associated with severe combined immunodeficiency (SCID).

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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