Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Blue 660-120S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine5/5.5/7
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780
• NovaFluor Blue, Red, UV, Violet, Yellow series
• Request custom conjugation

Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 complexes with CD132 (common gamma chain) to form the IL-7 receptor (IL-7R), a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells. IL-7 binding activates signaling through tyrosine kinase pathways including Jak/STAT, essential for lymphocyte development and T-cell homeostasis.

CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). These cells, characterized by low CD127 expression, are enriched for regulatory T populations expressing Foxp3 and exhibiting suppressive activity.

Binding of the eBioRDR5 antibody to PBMCs is blocked by recombinant human IL-7. Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human PBMCs at 5 µL (0.6 µg)/test (100 µL final volume).

Note:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer (Product # C001T06F01) is required for optimal staining. Add 5 µL per sample or per 100 µL cell suspension (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.
Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD127 (Interleukin-7 receptor, IL-7R) is a glycoprotein regulating lymphopoiesis.
The active receptor is an alpha/gamma chain heterodimer; the gamma(c) chain mediates signal transduction, while the alpha chain determines specific signaling events.
CD127 promotes proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+/CD8+ T cells.
Dysfunction of CD127 is associated with severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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