Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), NovaFluor Blue 610-70S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

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Product Specifications

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Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet/Yellow series
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine series
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright series
• NovaFluor Blue/Red/UV/Violet/Yellow series
• Request custom conjugation available

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Product Specific Information

The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 forms a complex with CD132 (common gamma chain) to generate the IL-7 receptor (IL-7R). It is a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells.

Binding of IL-7 activates tyrosine kinase pathways including Jak/STAT, essential for lymphocyte development and T-cell homeostasis. CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs), making it a useful marker for identifying true T reg populations enriched for Foxp3 expression and suppressive activity.

Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7 (Product #14-1079-80).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Product #C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread impacts.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

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Target Information

CD127 (Interleukin-7 Receptor alpha, IL7R) regulates lymphopoiesis through its alpha/gamma chain heterodimer receptor complex. The gamma(c) chain activates signal transduction, while the alpha chain mediates specific signaling events. CD127 promotes proliferation of precursor B cells, thymocytes, and mature T cells.

Dysfunction of CD127 is associated with severe combined immunodeficiency (SCID) and T cell negative/B cell negative/NK positive SCID.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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